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A total of 84 mutants of a three-domain zinc finger protein have been analyzed for their DNA-binding specificity.
One way to alleviate this problem is to find more stable mutants of a membrane protein of interest.
Conversely, reverse genetic strategies focus on the mutants of a gene of interest by observing the phenotype so as to determine the gene function (Wang et al., 2011).
In Arabidopsis, mutants of a member of the same family, SCRAMBLED (SCM), are affected in root hair specification (Kwak and Schiefelbein 2007).
This is illustrated by results from modeling of the interaction of the wild-type and 17 single and double mutants of a camel antibody specific for lysozyme.
Little is known about mechanisms responsible for the temperature-sensitive (ts) phenotype, or of the transferability of ts mutants of a specific gene between organisms.
Similar(19)
Primers for C-terminal mutants of A-RAF contained SmaI and HindIII respectively.
Deletion mutants of A-RAF were generated by insertion of PCR products into the pUG36 plasmid.
In the case of N-terminal mutants of A-RAF, BamHI and HindIII recognition sequences have been attached to the sequence of forward and reverse primers respectively.
Define ν i, m = ∑ j W ji, m to be the fraction of m-mutants of a protein in bin i that still fold, and let V m be the matrix with diagonal elements given by V ii, m = ν i, m and all other elements zero.
The analysis reported here, reveals that different mutants of A. fumigatus have a major influence on the ability of macrophages to adhere and to phagocytose the respective conidia.
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