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Previously, we constructed a S. cerevisiae strain accumulating approximately 120 mg/g-Dry cell weight RNA using suppressor mutants obtained from rrn10 disruptant (Chuwattanakul et al. 2011).
In this work, we have analyzed the stability of protein mutants using two different datasets of 1396 and 2204 mutants obtained from ProTherm database, respectively for free energy change due to thermal (ΔΔG) and denaturant denaturations (ΔΔGH2O).
In conclusion, our results constitute an important step on the genetic optimization of non-domesticated oleaginous species for industrial purposes, despite the increase on fermentative performance of mutants obtained from UV-irradiated L. starkeyi DSM 70296 was not so evident than those reported in these other studies (Wang et al.2009; Nishiushi et al.2012).2012
Glycosynthase mutants obtained from Thermotogamaritima were able to catalyze the regioselective synthesis of aryl β-d-Galp(1→3 -β-d-Glcp and aryl β-d-Glcp-(1→3 -β-d-Glcp in high yields (up to 90 %) using aryl β-d-glucosides as acceptors.
If our results were to be duplicated using aspartate mutants obtained from site directed [39] or random mutagenesis [33] this would strengthen the tentative conclusions we draw below.
For introduction of the mutated alleles, pBH01 was digested with BamHI/BstAPI and the backbone was ligated to a fragment carrying most of the coding sequence and the terminator of the WSC1 cysteine mutants obtained from the pUG6 derivatives described above, to yield pJJH1169 (WSC1C4,5A), pSK401 (WSC1C6,7andand pSK402 (WSC1C8A).
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'Meh0106' is a homozygous FHB-susceptible mutant obtained from M2 progenies of Wangshuibai with dry seeds treated with 0.35% (w/v) EMS.
n=4 wild type + 4 Cdkn1c mutant, obtained from two litters (2+2 for each litter).
Interesting to note is that the mutant obtained from this study also overexpressed eight oxioreductases that can use NADPH as an electron donor.
This set also included parental genotypes of published mapping populations, namely JI281, JI399 and JI15 [ 45], cv 'Terese', K586 which is a mutant obtained from Torsdag [ 46, 7] Champagne [ 7, 20], JI296 and DP [ 12].
The receptor mutant, obtained from Dr. Kazlauskas and collaborators, lacks the extracellular ligand-binding domain and is therefore unable to bind or be activated by proper PDGF ligand(s) or other signaling molecules [16].
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