Despite the higher levels of ethylene in Δ argR/Δ gltBD, this strain also displayed peak production between OD600 0.2 and 0.3 similarly to the single mutants, likely due to the depletion of O2 in the head space (Fig. 1b).
Moreover, CIT1 upregulation, combined with downregulation of IDH1, an enzyme catalyzing the subsequent step of the TCA cycle, is the most prominent metabolic feature of the studied mutants, likely leading to citrate accumulation.
The increase in chromosomal breaks in Slbp15 mutants likely contributes to the increased LOH observed in these mutants.
Our results reveal an absolute requirement for Aldh activity in pancreas development and demonstrate residual Aldh activity in aldh1a2um22 and aldh1a2i26 mutants, likely due to maternally contributed Aldh1a2.
Expression analysis of rps19 mRNA showed >90% reduction in the rps19 homozygous mutants likely due to nonsense-mediated decay of mRNA.
However, since then no phenotypes in ion channel regulation have been reported for recessive single pp2c insertion mutants, likely due to redundancy in these PP2Cs.
The first application of this method by Duffy et al. (1998) uncovered one of the first round egg mutants, likely corresponding to the βPS integrin gene myospheroid.
Mobile receptors detected by FRAP in frm-3 nlg-1 double mutants likely correspond to extra-synaptic receptors on the cell surface (which mediate muscimol-activated currents).
The genes in clusters 1A, 1B, 2A, and 2B show little to no response in the mutants at 72 h, suggesting that the disrupted loci in these mutants are likely to be involved in the early steps of bradyzoite differentiation.
Thus many mDEL1 mutants are likely to grow faster under selection than that estimated for cells with a single copy of the mutant purHD-lac locus.
