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Forward genetic screens for round egg mutants, like the one described here, provide a likely avenue for these discoveries.
In support of this notion, both mutants, like the wild type optineurin, were found to be ubiquitinated (data not shown).
Moreover, gait analysis showed that co-IgNrg1 mutants, like the Bace1 mice described above, suffered from pronounced deficits in forelimb/hindlimb coordination.
On the other hand, some family members or individuals carry additional genetic mutants like the P56S-VAPB which are insoluble in buffers.
In Arabidopsis thaliana, some circadian mutants like the toc1-1 allele were first thought to exhibit dosage sensitivity (Millar et al. 1995), but this was later attributed to a dominant-negative mutation in the repressor domain of the protein (Gendron et al. 2012).
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In the gonadal somatic cells, however, Piwi remained localized to the nucleus in the double mutants, like those in the heterozygous controls.
In the ΔHU mutant-like situation, the oscillations are symmetric, and for precisely tuned values of the parameters, it is even possible to obtain oscillations with a period of half the helicity of DNA (Figure 3C, blue curve).
The patterns of the mutation effects in various E730 and E734 mutants, like that of the E650 mutations, are not straightforward for making such a conclusion.
However, considering the extended time to death, it is likely that the ahpC mutant, like the previously described katG mutant [13], has an impaired rate of replication in target organs indicating an important function for AhpC.
The T686A mutant, like the D664A mutant, was unable to restore either checkpoint signaling (Fig. 2B, 2C) or chromosome alignment (Fig. 2D).
The cbbLS::kanr mutant was not expected to grow in air because this mutant, like the cbbL insertion mutant constructed by Baker et al. [10], did not produce the carboxysomal Form I RubisCO (Figure 4) and relied instead on utilization of the available intracellular inorganic carbon pool by its Form II RubisCO.
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