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The zebrafish mutant line cloche provides genetic evidence since homozygous mutants lack both hematopoietic and vascular endothelial cells but organogenesis is otherwise normal [11].
nac mutants lack both larval and adult melanophores.
In zebrafish, fgf3 mutants lack both hypothalamic axonal projections and the hypophyseal artery and capillaries.
neurogenin1 mutants lack both afferent axons and peripheral glia (Andermann et al., 2002; López-Schier and Hudspeth, 2005) but develop small, supernumerary neuromasts with normal hair-cell morphology, planar polarity, and capacity for regeneration (Nagiel et al., 2008).
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Surprisingly, even double mutants, lacking both mitochondrial forms of SOD, show no reduction in lifespan.
In addition, mutants lacking both N-terminal 370 or 467 residues and the C-terminal 99 amino acids were generated (371-Ynd1-531 and 468-Ynd1-531 respectively).
To find a possible explanation for the high amplitude resetting observed in mutants lacking both Rev-Erbα and Per1, we examined induction of clock genes in response to light.
This is in agreement with a previous study performed by our group in which mutation of the corresponding sites in rat HSL (Ser563 and Ser565) was found to have no effect on the activity [9], and also with another study on rat HSL reporting that deletion mutants lacking both Ser563 and Ser565 retained full enzymatic activity [27].
In double mutants lacking both pools, immobilized synaptic UNC-49 receptors and mIPSCs are eliminated.
In double mutants lacking both HDACs, the expansion phenotype was even more pronounced.
Only double mutants lacking both CgSod1 and CgYap1 were efficiently killed by primary mouse macrophages.
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