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Exact(8)
Cautionary notes: The number of intestinal SQST-1 aggregates in rpl-43 mutants is very sensitive to a variety of stresses.
Analysis from Ji's group demonstrated that the lethality observed in the Kdm2 mutants is very likely due to second-site lethal mutations.
Unfortunately, this has proved to be very difficult due to technical reasons: growth of GARP mutants is very sensitive to inhibition of sterol biosynthesis (e.g., shown by the synthetic lethality of vps52∆erg3∆, Figure 4 figure supplement 1).
An important notion is whether a mutant colony expands and persists inside an organ for a long time, which, for large values of N and for advantageous mutants, is very close to the probability of invasion.
Interestingly, the reduction in food intake we describe in long-lived eat mutants is very close to the reduction in food intake necessary to extend lifespan in mammals (Taormina and Mirisola 2014).
For example, the growth plate phenotype of Mmp9-Mmp13 double mutants is very severe compared to the growth plate phenotype of either Mmp9-or Mmp13-null mutants (Stickens et al., 2004; Vu et al., 1998).
Similar(52)
Although these mutants were very efficient to convert low amount of exogenous DX, at higher DX levels they performed only slightly better.
In comparison to the original activity of pepsin, the kinetic constants of these mutants were very low with Km values of 4.10 and 2.10 mM, and k0 values of 22.2 and 18.0 min−18.0
Resistant mutants are very rare, except for vancomycin-resistant Enterococcus.
We have also shown that serotonin mutants are very defective in their time allocation.
Defects in synaptic growth as described in TBPH mutants were very often associated with alterations in synaptic bouton shape.
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Justyna Jupowicz-Kozak
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