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One unique feature among these mutants is that D316G is directly involved in interactions of the monomers, while the other two mutations indirectly affect the interactions of the EDA homotrimers.
One major difference between the two mutants is that tko 25t has an essentially normal lifespan (Chen et al., 2012), whereas that of sesB is drastically shortened (Fig. 1D).
Since the main difference between these mutants is that aos also lacks OPDA, the contrasting results for cat2 aos and cat2 jar1 could indicate distinct roles for OPDA and JA in H2O2 induced cell death.
DOI: http://dx.doi.org/10.7554/eLife.03558.011 A striking difference between Marf mutants and Drp1 mutants is that loss of Marf severely affects NMJ morphology whereas loss of Drp1 does not affect NMJ development.
The main difference between compact folds, wild-type and C3A/C26A, and cavity mutants is that the inversion point is powered from ∼3 kbar to 1.5 kbar for F110S and <0.1 kbar for I7S, meaning that in the latter species pressure-induced internal hydration dominates very early over any compaction of the globular fold resulting from the reduction of internal free volume.
The second proposed function for IDH mutants is that they gain the neomorphic activity to reduce α-ketoglutarate to 2HG [6], [15, [15].
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A starting premise for the investigations on the PEX13 brain mutants was that brain dysfunction would be sufficient explanation of the major phenotypic characteristics of these mutants.
The advantage of using this particular RNA-binding mutant is that Flag-TDP-43 F4L is unable to downregulate the endogenous TDP-43 levels by the autoregulation mechanism (6).
The rationale for using the F219L mutant was that Phe219 has been replaced with a smaller residue (Leu), which could make the cytoplasmically closed state less stable.
One potential explanation for the male precedence defect in comp-1 mutants was that sperm do not undergo proper spermatogenesis or spermiogenesis necessary to mature into functional sperm.
A common feature of the bal1 variant and the dms4 mutant is that both are likely to harbor widespread epigenetic alterations in their genome.
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