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Finally, four mutants, Sm5-V1, Sm2-V10, Sm7-V12, and Sm7-V12, were identified in the ARTP breeding process, and the TGase production of these mutants is shown in Table 3.
To illustrate the potential of microplate technology as a high-throughput phenotyping tool, a screen for tomato metabolic mutants is shown where 24 enzymatic traits have been measured in more than 1500 samples within only 1 month with a relatively small investment.
The structure of these five mutants is shown in Figure 3A.
The expression profile of the genes surrounding the insertion point in each of the mutants is shown in Table S1.
The range of DD outgrowth defects observed in ju89 mutants is shown in Figure 3 (A D).
The network that emerged from the screen and validation as being most consistent with the phenotypes of the deletion mutants is shown in figure 7.
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Schematic of LbCpf1 and AsCpf1 DNase-dead mutants was shown in Fig. S1A.
The genotypes of WT and the overexpressing mutants are shown in Fig. 5a.
In addition, four mutants are shown to significantly stabilize Raf RBD native structure.
These genes were amplified by the primers cueO-F and cueO-R, and gene mutants are shown by vertical bars.
The average heading dates of the wild type and the z3 mutants are shown by closed and open arrows, respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com