Interestingly, although most rice early flowering mutants are regulated by photoperiod, measurement of flowering time under long-day treatment (13.5 h light/10.5 h dark) showed similar responses of el1 and WT plants, indicating that the function of EL1 is independent of the photoperiod.
To assess whether the N-glycosylation-deficient mutant is regulated by CCE, in vivo cAMP accumulation experiments were performed in HEK-293 cells transiently expressing WT AC8 or the triple N-glycosylation mutant (AC8N814Q/N818Q/N855E)(Fig. 4 D ).
Thus, most of the genes up-regulated or down regulated in one mutant were regulated in the same way and with similar strength in other mutants in which their expression was affected.
Here, we demonstrate that the stability of stress-induced wild-type and mutant p53 is regulated by the type I phosphatidylinositol phosphate kinase (PIPKI-α (also known as PIP5K1A)) and its product phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5 P2).
The one gene that did show increased RNAPII occupancy in the fogsk8 mutant, gadd45b, is regulated by multiple important cellular signaling pathways including NF-kB and JNK [47] and is involved in zebrafish somitogenesis [48].
10.7554/eLife.06132.036 Figure 7. SOX2 expression in EGFR-mutant cells is regulated by FOXO6.
Therefore, K159 mutant pVHL is regulated by another mechanism in addition to ubiquitination.
The hypothesis that the activation of the mutant pseudoexon is, in fact, caused by creation and/or strengthening of an SF2/ASF binding ESE was confirmed by our knock-down and overexpression experiments, which showed that inclusion of the mutant pseudoexon is regulated by the cellular amounts of SF2/ASF.
Furthermore, the binding kinetics of 15 mutants revealed that binding is regulated by long-range interactions, which can be correlated with the structural rearrangements resulting from peptide binding.
Unlike ARF4, KAN1 expression was significantly reduced in quadruple mutants, suggesting that KAN1 is regulated directly and positively by the YABs.
