An advantage of parallel analysis of barcoded mutants is that mutations causing deleterious or weak phenotypes can be efficiently detected.
The dose-response curve of this truncated rhChM-I mutant was found to be parallel to that of the ChM-I cyclic peptide.
Three further CHC mutants were tested in parallel.
Cultures in 16 such replicate plates, each with independently generated double mutants, were grown in parallel.
In addition to 384-well mutant arrays, mutant pools of 1800 mutants are available for parallel analysis.
The identification of auxotrophic mutants was performed by parallel plating on minimal media MM1 (MMYE without yeast extract [ 88]) and TSB complex media.
The γ-secretase activities in the presence of wild-type β-arrestin1 or the mutants were measured in parallel (Supplementary information, Figure S2D).
When all four mutants were analysed in parallel, the degree of retention was directly proportional to the length of the polymers and the severity of the clinical phenotype caused by each mutation.
After confirmed by PCR and sequencing, the mutants were grown in parallel with wild type Synechocystis in both normal BG11 medium and the BG11 medium supplemented with 0.25% (v/ v) butanol.
After confirmation by PCR and sequencing analysis, the mutants were grown in parallel with wild type Synechocystis sp. PCC 6803 in both normal BG11 medium and the BG11 medium supplemented with 1.5% ethanol.
