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Since a modest increase in viral protein synthesis in these mutants is observed despite no increase in viral RNA levels, an appealing hypothesis is that 2C has some involved in translation, and that R55W might enhance protein synthesis modestly but to a level sufficient to contribute to enhanced cytopathology.
An increase in the maximum growth rate of both mutants is observed during the by-product exploitation period (Additional file 3: Supplement FA).
An unusual laddering below control HTT in the presence of SUMO-2, but not in the presence of lysine mutants, is observed for this construct; however, the laddering is not detected by anti-GFP, and its significance is not clear.
In addition, mitochondrial function is affected by ALS mutations in TDP-43: an inverse correlation between respiratory activity and toxicity of the mutants is observed in yeast (50), while the mitochondrial membrane potential is reduced in motor neurons transfected with a TDP-43 mutant (46).
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The mutants were observed to be freeloaders that exploited the bacterial chow provided by the normal-skinned farmers.
Changes of the height, fresh weight, dry weight, and culm width of the five DongnongV7 tall mutants were observed in the progeny individually.
A similar 104 105-fold enhancement in the reactivity of factor Xa with prethrombin-2 and the HAT mutants was observed in the presence of the cofactors Va and heparin, respectively.
The colony morphology of the mutants was observed after 1 week of culture.
The skn7 mutants were observed to be more susceptible to reactive oxygen species in vitro and were significantly less virulent than the wild-type strain and a reconstituted strain as measured by cumulative survival in the mouse inhalational model.
Since the growth of cyclohexane-tolerant mutants was observed after 48 h, the aqueous medium phase was spread on LBGMg agar medium.
Since no difference in the enzyme variants from the mutants were observed, detailed description of each individual enzyme variant is not described here, and further suggested to refer our recent report (Kumar and Parikh 2015).
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