Sentence examples for mutants into the from inspiring English sources

To determine the extent to which each of these genes alters FLP-7 secretion properties, we crossed the respective null mutants into the FLP-7mCherry secretion line.

Internalization of S. aureus eap and sae mutants into the MAC-T epithelial cells was significantly decreased compared with the wild-type counterparts.

However, surprisingly, we found that forcing the K122 acyl mutants into the mitochondria with an IMS-targeting tag did not recover their ability to suppress respiration.

For UTI89, we inoculated 107 CFU of the WT strain or one of the secretin mutants into the bladder by trans-urethral catheterization.

In order to answer this question, we microinjected SB cheater mutants into the dendrites of swarming WT P. aeruginosa communities (Figure 3).

These CEN-based plasmids were used to obtain the data in Fig. 3B,C and D. To integrate mad3 mutants into the genome, we excised a PmlI-EcoRI fragment (containing CEN4, ARS1 and TRP1) from these mutant plasmids and replaced it with a PmlI-EcoRI fragment from YIplac204 (containing TRP1) to generate derivatives that could be targeted to the TRP1 locus following linearization with BsgI.

As an immediate practical application we report the expression of a complete functional monoclonal antibody expressed from a single polypeptide, which was cleaved by our TEVp mutant into the two antibody chains and secreted as an assembled and functional molecule.

We then asked the question whether the interaction between SPT7L and TAF8 would be maintained and thus SPT7L would take the TAF8 mutant into the nucleus (Fig. 3A).

We then inserted a GFP Tyr151TAG) mutant into the same plasmid under the Mtb hsp60 promoter to afford plasmid pSMT-MjtRNA-GFP151TAG (Figure 1A).

Toward this end, we insertionally inactivated BA3443, BA0672, and BA1295 in a ΔSterne/Wip4 background and inoculated each mutant into the worm microcosm.

An equally important factor that likely impacts the additive effects of two activating mutant alleles is the combined incorporation of each mutant into the sarcomere given the central role thin filament stoichiometry and cooperativity plays in the regulation of contraction [40].