Nogo transcripts are up-regulated in NgR mutants, indicating that NgR regulates Nogo in vivo.
Loss of Tfeb function is sufficient to restore myelination in RagA mutants, indicating that hyperactive Tfeb represses myelination.
AR-12 treatment did not affect the high protein levels of RS6 or P-RS6 seen in the lkb1 mutants, indicating that they are unsusceptible to PI3K pathway inhibition.
Indeed the levels of bacterial growth in FLS2K/R plants were comparable to that in fls2 mutants indicating that SUMOylation is vital for FLS2 dependent antibacterial immunity in Arabidopsis.
qPCR results showed that the induction of NRT1.1 by nitrate was notably decreased in the nlp7 mutants, indicating that NLP7 affects the response of NRT1.1 to nitrate (Fig. 1c).
Interestingly, our data revealed that the nitrate reductase activity was notably depressed in the chl1 mutants, indicating that the reduced nitrate content in the chl1 mutants may result from both decreased nitrate uptake and reduction.
Infection of macrophages by B. abortus was stimulated by light in the wild type but was limited in photochemically inactive and null mutants, indicating that the flavin-containing histidine kinase functions as a photoreceptor regulating B. abortus virulence.
First, we screened mutants for 18 TRP channel genes (including all TRPV orthologs) and found only minor defects in heat avoidance in single and selected double and triple mutants, indicating that other genes are involved.
Remarkably, these effects were absent in TASK-3 mutants indicating that TASK-3 is either directly involved in the mechanism of this drug's action, or participates in parallel pathways that achieve the same effect.
After replacing aromatic residues, the affinity towards the CMC increased in the case of the W139A and W53A mutants, indicating that the aromatic residues play a key role in substrate affinity and binding.
Given that DA transport efficiency by DAT is largely governed by surface DAT expression56, our results show that mutations of these residues do not alter either cell surface DAT expression or [3H]WIN35,428 binding sites in intact cells expressing these mutants, indicating that the altered Vmax in these mutants is not due to changing surface DAT expression.
