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Figures 4D and E analyze the banding pattern of the cytoplasmic receptor in cells transfected with the Δ1 and Δ2 receptor deletion mutants indicated in C. Interestingly, the cytoplasmic mutant 355 503 (Δ2) is not sumoylated (Fig. 4E) while the fragment spanning aminoacids 355 531 (Δ1) is sumoylated (Fig. 4D).
Competitions between bacteriophage Qβ virus clones (Qβwt and the site-directed mutants indicated in each experiment) were carried out by mixing equal amounts of each competitor virus (10 or 10 pfu from each of them) which were used to infect 10 bacteria in a final volume of 10 ml.
Most notably, it is found that for two of the EmrE mutants (indicated in dashed boxes) the translocon charge mutations dramatically shift the slowest-flipping loop position in the EOT ensemble from being primarily cytosolic to being primarily periplasmic, suggesting that the dominant topology for these EmrE mutants will be similarly reversed by the translocon charge mutations.
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The top part of each panel shows CPMG profiles for pWT apoSOD12SH, with the corresponding curves for the A4V mutant indicated in the bottom.
To determine them, liquid cultures containing 10 bacteria growing in exponential phase were inoculated with 10 pfu of the site-directed mutant indicated in a final volume of 1 ml.
Furthermore, analyses of the β-cateninlof mutants indicated that in addition to the rhombomere 1, also many other domains of Fgf8 expression are dependent on β-catenin activity.
DOI: http://dx.doi.org/10.7554/eLife.07424.011 Analysis of Red1 binding in hop1Δ mutants indicated that peak heights were affected in a non-uniform manner.
Gaps in the arterial wall and the presence of blood cells outside of vessels in mutants indicated that compromised vessel structure led to hemorrhage.
UNC-40 was required for the excess protrusion in unc-5 mutants, indicating that, in repelled VD growth cones, UNC-6 and UNC-40 control both pro- and anti-protrusive activity.
Previous studies showed that expression of RLC-E20E21 could rescue the rough eye phenotype of Drosophila Citron mutants indicating an in vivo role for Citron in RLC phosphorylation [28].
Protein expression and purification protocols for Kv7.2 helices AB and the helices ABCD fused to GST (GST-AB and GST-ABCD), the deletions and mutants (as indicated in the figures), and for CaM have been described in detail elsewhere36,37.
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