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Other eIF4E mutants, including those with a stop codon in exon 1 of Sl-eIF4E2 and a stop codon in exon 2 of Sl-eIF iso 4E, Sl-eIF iso 4Esceptible to potyviruses.
Surprisingly, when live cells were observed, all of the mutants, including those that showed diffuse nuclear staining in fixed cells, gave punctate localization indistinguishable from wildtype (Fig. 1, right).
MPA sensitivity has been shown to correlate well with upstream TSS shifts of a subset of Pol II mutants, including those tested here (Braberg et al. 2013).
We tested most of the white mutants and some of the blue mutants, including those that had a wild-type response to the CYC1 and SNR13 terminators.
NKX2-5 mutants, including those with a crippled homeodomain, bound hundreds of targets including NKX2-5 wild type targets and a unique set of "off-targets", and retained partial functionality.
Microarray and real-time PCR analysis showed that the expressions of several cytokinesis related genes were up-regulated in the mutants, including those of ace2+, agn1+ and eng1+ (Table 3 and Figure 5B).
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Transporter genes expressed higher in the rpoS mutants included those for spermidine/putrescine (potABCD), glycine/proline (proWXY), and Zinc (znuABC).
Unexpectedly, every TCR mutant, including those in CDR1 and CDR2, retained remarkable peptide specificity.
Two pairs of mutants, including the I317C-G361C that remarkably stabilized the receptor (Fig. S5A), were included in the final crystallization construct.
RNAi inactivation of lsy-2 in SynMuv mutants, including the SynMuv B mutants lin-35, lin-9, lin-15B, and hpl-2 and the SynMuv A mutants lin-8, lin-15A, lin-56, and lin-38, caused no SynMuv phenotype.
The system was validated using a panel of HCV mutants, including the NS3 protease inhibitor drug resistance mutants R155K and T54A.
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