On the other hand, all the mutants, including the single H3-S57E mutant, displayed an additional 4 hour G2/M delay, indicating that all the mutants were unable to complete mitosis efficiently.
Results showed that five mutants, including three single (rcsB, rcsC, rcsD mutant), one double (rcsBD), and one triple mutant (hrpXYS), were non-pathogenic on immature pears (Table 3).
DOI: http://dx.doi.org/10.7554/eLife.00726.021 To test our prediction, we generated a series of KLF4 mutants, including three single (i.e., R458A, R458K, D460A), one double (R458A::D460A) mutants, and one truncation (Δ432).
Selected enzymes, including single amino-acid mutants constructed following evolutionary analyses, as well as a 6 truncated enzyme, which may be useful to explore hypotheses related to the evolution of the 8-fold, were synthesized and biochemically characterized.
We constructed a set of single-gene and double-gene mutants, including the conditional single gene mutants of P MET3-MMS22, P MET3-MRC1, P MET3-TOF1, and P MET3-CSM3, as well as the double-gene mutants of the aforementioned genes repressed together with MMS22.
To determine the influence of the N-terminal amino acid of enteropeptidase light chain (EKL) on the enzymatic activity, we constructed various mutants including 17 different single amino acid substitutions and three different extensions at the N-terminal end.
Consequently, SInv and 5 mutants including three mutants with single point substitution [Mut1 = P152 V, Mut2 = S85 V and Mut3 = K153F ], one mutant with two points [Mut4 = S305V-N463 V] and one mutant with three points [Mut5 = S85V-K153F-T271 V] were developed via site-directed mutagenesis and produced using Pichia pastoris as the host.
Observation of meiotic nuclear morphology identified 33 clones exhibiting abnormal nuclear morphology, and backcross analysis identified four single mutants including E46.
As a minimum, this should include a full characterisation of the mesenchymal phenotype in each of the mutants, including the Fgf9 and Fgf20 single mutants for comparison.
Three single-residue mutants, including hPrPC M166V), hPrPC S170N) and hPrPC R220K), show variations in the length and quality of definition of helix 3 [41].
