Sentence examples for mutants in single from inspiring English sources

All mutants showing an RNAi phenotype were revealed for identity and then crossed with ced-3 lf) ced-3 lfin single or in comutantson and tested for enhancer phenotypes.

Our analysis demonstrates the power of barseq screening to robustly provide growth-rate phenotypes for dozens of mutants in single mice, and opens up the possibility for large-scale reverse genetic screens for multiple areas of Plasmodium biology.

Unfortunately, the high demands of animal experiments complicate the analysis of many conditional knockdown mutants in single-strain infections.

We also tested the flgE mutant in single infection studies.

Furthermore, we show that experiments can be multiplexed and easily scaled for the identification of multiple individual mutants in a single sequencing run.

Although gene redundancy may affect the outcome of a screen, it should be noted that mutants affected in single catalase or other antioxidant activities do show an oxidative stress phenotype.

The development of yeast deletion strain libraries has allowed researchers to screen thousands of individual gene mutants in a single experiment to identify all mutants that have a particular phenotype.

Analyses of the ICL knockdown mutant in single-strain infections confirmed this and demonstrated that silencing of ICL during chronic infections impaired persistence of Mtb to the extent that the pathogen was cleared from the lungs of most mice.

However, even though complementation of icl-TetON in vitro and in vivo was incomplete, the analyses of this mutant in single-strain and multi-strain mouse infections demonstrated that ICL activity is not only essential for Mtb to establish an infection in mice but also to persist during chronic infections (Figure 10).

For example, the two genes YDR342C/HXT7 and YDR343C/HXT6, which encode glucose transporters and are involved in glucose signal transduction pathways [ 13, 14], were up-regulated to 3-4 fold in double mutants yet none in single mutants.

Advances in DNA synthesis and next-generation sequencing technologies have enabled the assessment of millions of mutants in a single experiment, providing new opportunities to study allostery.