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DltA mutants in several bacterial species have been shown to have a greatly increased susceptibility to AMPs.
We are currently analyzing mutants in several putative EHR transcription factors for their transcriptional pattern and phenotype in the defined hypoxic model.
No difference in virulence was detected when comparing two prevalent CA-MRSA strains, LAC (USA300 lineage) and MW2 (USA400 lineage), to their respective isogenic PVL knockout mutants in several mouse models, including subcutaneous abscess, sepsis and pneumonia models [10] [13].
In addition to targeted gene disruption by homologous recombination, ATMT has been successfully exploited for large-scale forward genetic screens to create insertional mutants in several pathogenic species, including Magnaporthe grisea, Cryptococcus neoformans, Colletotrichum lagenarium, C. higginsianum and Leptosphaeria maculans [24] [32].
Later studies showed the potential of BSA combined with SFP genotyping arrays in successfully mapping genes to mutant phenotypes in more complex genomes, such as Arabidopsis [3], [4] and the technique has been used to map mutants in several species [5] [10].
The survivor DM lines exhibited similarities to late generation trt-1 mutants in several aspects.
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Our detailed analysis of the ΔC3GMS mutants resulted in several important findings.
Mutants defective in several surface proteins previously shown to be involved in host recognition were immobilized and the ability of osteoblasts to adhere to the bacteria was measured.
The axonal phenotypes seen in RSK mutants differ in several respects from these phenotypes.
First, some compensation mechanism, for instance mediated by other Ctp proteins, may couteract ctpC deficiency; this may be addressed using mutants inactivated in several P-type ATPases, wich is ongoing in the laboratory.
A number of VGSC mutants found in several human diseases have been found to be trafficking-deficient and may give insights into key protein regions/domains important for the regulation of VGSC trafficking (Table 1).
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