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The experimental details for a high‐throughput microarray‐based screening technique for both detecting and mapping Tn5 insertion mutants in parallel within a library are presented.
Using a sensitive microarray-based strategy [25], we then quantified the change in prevalence of different transposon insertion mutants in parallel.
Recently, various studies have examined multiple Bbs mutants in parallel for additional phenotypes.
Simultaneous survivals were then conducted for multiple mutants, in parallel with their isogenic wild-type controls.
We next screened the 2847 prototrophic deletion mutants in parallel for altered CLS during quiescence in the absence of nitrogen.
Taken together, these data strongly suggest that barcode counting can be used to phenotype large numbers of mutants in parallel.
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To rule out any other possible glycine residues that might be involved in the protein cleavage, four additional glycine residues (Gly181, Gly191, Gly196 and Gly206) were mutated and the corresponding mutants tested in parallel.
' Thus, mechanistic insight into the reactions that activate checkpoint signaling at the kinetochore and testing the effect of KNL-1 microtubule binding on these reactions as well as elucidating whether KNL-1 mutants participate in parallel to or in the same pathway as dynein in checkpoint silencing are important future goals.' The results of the classification analysis are presented in Figure 1.
Mutation rates can be calculated by using the distribution of mutant numbers in parallel cultures from a Luria Delbruck fluctuation assay (Luria and Delbruck 1943; Rosche and Foster 2000, 2006).
For this reason, we also generated a second insertional mutant collection in parallel by transforming N. gaditana cells by electroporation and selecting transformants on plates containing zeocin [ 35].
The size of oocysts was measured from wild-type and mutant parasites in parallel using pictures of midguts containing GFP-positive oocysts recorded using a CoolSNAP HQ (Photometrics, the Netherlands) digital camera on a Leica DMR microscope.
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