In fact, forward genetic screens have generated zebrafish Titin mutants, although in only one case is the position of the mutation known (Xu et al., 2002; Steffen et al., 2007; Myhre et al., 2014).
They could be key candidate genes representative of the reprogramming machinery involved in the control of shoot growth and development in the two mutants, in which only grain yield, but not leaf size or leaf number, was affected [ 13].
Interestingly, DT40 cells defective in xrcc3/rad51d, in which both the BCDX2 and CX3 complexes are impaired, exhibit higher sensitivity to camptothecin and cisplatin than rad51b/rad51d-double rad51b/rad51d-doublely BCDX2 comutantss dysfunctinnal (71).
The different cell types were deletion mutants in which only the signaling branch through Sln1 activation or Sho1 activation was active and the wild type cell in which both branches were active (Fig. 1).
To examine the SUMOylation status of each site, we constructed hemagglutinin (HA -tagged PML4 mutants in wHA -taggeda single lysine is available for SUMOylation, namely K65/160R, K65/490R, and K160/490R.
The phosphoserine-mimicking cortactin mutant accumulated in only 21% of pedestals and showed a weak, diffuse pattern of localization in the cytoplasm and pronounced staining in the nucleus.
The majority (2492; 91.9%) of these Mtb mutants dysregulated responses in only one or two of the IR clones (1885 and 607 Mtb mutants, respectively).
For example, the mutant in which only K48 is present but the remaining six lysines have been mutated to arginines is termed "K48 Ub" in figures, whereas the mutant in which only K6 is present but the other six lysines have been mutated to arginines is termed "K6 Ub".
YnaI mutants were recorded in only KCl solution by the same method.
We also tested a Δ6 20 mutant in which only this critical region was deleted from the N terminus [33].
