We cannot, however, explain why CDK9 S175D mutant in normal conditions is seen associated with 7SK snRNP (Fig. 7B, lane 3).
To study whether the untagged Cx40 Q49X mutant is also able to alter the localization of endogenously expressed Cx43, we expressed the Q49X mutant in normal rat kidney (NRK) cells.
Moreover, iron chelation further decreased the size of ZIPCO mutant liver stage and drastically decreased the growth of the wild-type liver stage to a size smaller than that of the ZIPCO mutant in normal conditions, clearly suggesting that liver stage express iron transport ability in addition to ZIPCO.
Moreover, the nerve fibers, consisting of retinal ganglion cell axons, are disrupted in the retina and also are much thinner in the retina and optic nerve head in mutants than in normal controls, suggesting that the retinal ganglion cells axons are degenerative in mutants as early as 3 weeks.
Still, we used qPCR to measure RFeSP and Blw levels in delg mutants, grown in normal or low yeast food.
We also used a mutant specific antibody (Supporting Information Fig S1D) and found mutant huntingtin in normal residual tissue and invasive cells with a strong nuclear staining.
However, observations from a few studies do suggest otherwise: Sussel and colleagues reported that Nkx2. 1 mutants, in which normal MGE tissue fails to form, show a 50% reduction in cortical interneuron numbers relative to wild-type [ 95].
The critical roles for mutant TFs and/or PTKs to play in leukemogenesis, and the absence of mutant TFs/PTKs in normal hematopoietic cells, suggest that the two types of aberrant molecules may serve as ideal therapeutic targets.
OIS was identified in 1997 when overexpression of mutant Ras in normal rodent cells resulted in permanent growth arrest via p16ink4a and p53 [ 121].
