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To understand the 31 identified biofilm mutants in more detail, various aspects of biofilm matrix production and breakdown were investigated.
We have characterized E74 mutants in more detail as a proof of principle for our biochemical approach that identified LARK target RNAs.
To characterize the MinC-sensitivity of the ftsZ mutants in more detail we compared filamentation upon GFP-MinC/D overexpression of a strain expressing wild type ftsZ (in the same genetic background) with the strains expressing the ftsZ mutants.
To examine and quantify differences between the mutants in more detail, the infection process of leaf rust was studied microscopically after staining fungal structures with WGA-FITC [ 18].
To elucidate the mechanism underlying impairment of the Ras-MAPK pathway-mediated CL, we analyzed the locomotion behavior of let-60 lf) let-60 lfn mutantstain.
Yi and Richards reported destabilization of phenotypes in both the bal and cpr1 mutants in more than 10% of EMS-treated plants in the M 1 generation.
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We next sought to examine the turnover rate of the Pro3-1 mutant in more detail because of its pronounced degradation in the first assay.
Significance was tested by T-test; n ≥ 15, *: significantly different from the wild type (col), P < 0.01 To analyze the polysaccharide composition of the cell wall of the double and the triple mutant in more detail, a high-throughput characterization using Comprehensive Microarray Polymer Profiling (CoMPP; [ 27]) on rosette leaf and stem extracts was performed.
The levels of DSB formation in mec1Δ cells treated with HU were lower than those observed in the absence of HU, which we believe is due to relocalization of DSB factors in checkpoint mutants (discussed in more detail later).
We also analyzed the mechanism whereby the present mutation caused the generation of two different mutant transcripts in more detail by determining the order of intron removal.
However, cleavage in the U232C mutant results in more branching than hydrolysis product as in wild type, whereas cleavage in the U207C mutant results in more hydrolysis than branching product.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com