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We tested this by culturing eat-2 ad1116) eat-2 ad1116iquid bacteria using the BDR mutants

To evaluate growth of the pts and hprK mutants in liquid cultures, overnight cultures were diluted to an OD600 of 0.05 in 2YT complex medium and grown at 37°C with constant shaking.

The growth of these mutants in liquid defined media, but not in liquid complex media, was also severely impaired compared to a wild-type strain (Figure 2B and 2C).

The biofilm screen was conducted by growing each of the 4019 mutants in liquid synthetic complete medium with 0.2% glucose and ammonium.

The first involves competition between deletion mutants in liquid culture, while the second uses a robot to seed arrays of mutants on agar - in both systems the impact of drugs on the mutants' growth may readily be measured and the genes specifying drug carriers identified by the drug resistance of their cognate deletion mutants.

Similar(55)

However, melanized microsclerotial production was observed for V592 but not for vdgarp1 mutant in liquid Czapek-Dox medium at 6 to 9 dpc (Figure 6D and 6E).

Growth of the tatC mutant in liquid TY medium was slightly impaired (it had a doubling time of 4.5 h compared with 3 h for WT and the other mutants).

When we co-incubated the wild type and the Δ tpsA- tpsC mutant in liquid medium as described for E. coli[ 23], no growth inhibition of the mutant was observed independent of the media used (TSB, LB, or RPMI), co-incubation time (3 6 hours) or initial ratio of target and killer cells (ranging from 1 1 to 1 1000).

Meanwhile, eat-2 mutants grown in liquid culture actually have an increased metabolic rate, and the metabolic rate of eat-2 mutants grown on solid medium has not been evaluated [ 174, 179].

This growth defect did not provide sufficient biological material for GC analysis for loa1Δ mutants grown in liquid high-N YNB medium at 28 °C.

In addition, we also examined the expressions of HXT (a hexose transporter, CNB02680) and SNF1 in the C. neoformans MATα strains including three ssn8 mutants in YPD liquid medium.

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