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The cell wall destabilising agents Congo red, caffeine, and calcofluor white had no significant effect on the growth of the pmt mutants in either variety (data not shown).
Correctly determining the pathogenicity of potential mutants (in either genome) is critical to understanding mitochondrial disease.
Mutants in either mrpC or fruA fail to aggregate or sporulate.
Mutations affecting mitochondrial genome maintenance and translation were highly represented in sensitive mutants in either growth phase.
In addition, mutants in either mamK or mamJ have no magnetite crystallization defect but display severe magnetosome alignment perturbations.
Null mutants in either clpT1 or clpT2 do not show noticeable phenotypic changes from the wild type, while the double mutant is seedling lethal [ 21].
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We observed no attenuation for the Δafoch1 mutant in either model of infection (Figure 5B and C).
We never reported any mutant in either assay used for our non-mutant control DNA, indicating that our specificity is high.
Quantification of bZIP1 mRNA levels by RT-PCR, revealed that transcripts of bZIP1 are undetectable in the homozygous bzip1- 1 T-DNA mutant in either shoots or roots.
The inability to detect free cysteines for the P1040C/F1053C mutant in either assay suggests that an internal disulfide forms as predicted by the LigB-12 structure-based model.
Can the authors show that the interaction is important for PCVII to exit the ER? (i.e., identify a mutant in either TANGO1 or SLY1 that disrupts the interaction, and test whether this mutant is functional for PCVII ER export).
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