Moreover, gpn2 -2 showed synthetic lethality or synthetic growth defects when combined with mutants in the core RNAPII subunits RPB1 (RPB21 -1), RPB2 (rpb2 -6) or the transcriptional regulator ESS1 (ess1 -H164R), and gpn3 -1 also showed synthetic lethality with rpb2 -6.
In mutants of core components or of proteins involved in complex assembly, the SJ complex is not properly formed and the free GFP-linked proteins can diffuse rapidly to the bleached region (Oshima and Fehon, 2011).
We observed that Ago1 mutants exhibited stronger effects on eye development and embryonic viability than mutants in the other core factors.
Do they think the previous papers missed a disruption of MT orientation in core mutants in the distal region?
Interesting, both Zipper and Bazooka also lose asymmetry in core pathway mutants (supplementary material Fig. S4A,B), although Arm does not (Fig. 4F).
Thus, the WHSC1 −/− phenotype described here is remarkably similar to LIS1 cKO, but does not correlate with defects observed in core PCP mutants.
However, arguing against Src acting together with the core pathway, Src in part modulates E-cad by altering its transcription (Shindo et al., 2008), whereas in core pathway mutants we see no change in expression of a reporter of E-cad transcription (Fig. 4M).
In support of the view that the increased levels of E-cad in core pathway mutant backgrounds are responsible for the intercalation defect, we found that the dsh phenotype was suppressed by a reduction in E-cad gene dosage (Fig. 2G).
Analysis of the corresponding linker mutants provided results comparable to EspP, with transport inactive Pet mutants T573, A576 and F589 located within Domain 2 of our Pet model, and active mutants in the flanking β-helical core region (Fig. 4B).
We isolated multiple intragenic suppressors of the Cdc6-E224A or E224Q single mutants in the highly conserved 'DEMD' core region of the Walker B motif, that is, AAMD, AAAD, AAMA, NQMD, NQMA, and NQNM.
To experimentally address which state in the free exosome complex interacts with the cap, we designed a mutant exosome core in which the equilibrium between the two states is significantly altered.
