Moreover, this concept is applicable to predict which drug to use against a particular receptor mutant in case of e.g. personalized medicines, such as in case of the question which drug to use against a particular HIV genotype [7].
However, the extent of influence on the folding of protein was much lesser in these cases in comparison to the W377A mutant; in case of the V209D and D382A mutants, the synthesized protein was localized equally in the soluble and insoluble portions (Fig. S1).
Furthermore, analysis of dEAAT2 transcript levels revealed a down-regulation in TBPH null mutants in all cases examined (n = 4) when compared with w controls (Fig. 7F), which we also observed in all cases of glial-specific TBPH gain-of-function flies (n = 6) when compared with heterozygous repo-Gal4/+ controls (Fig. 7G).
In order to generate longer surviving mutants, in rare cases up to P24, a mixed genetic background was necessary.
Fitness was reduced in almost all the ΔmotAB strains, and these reductions were statistically similar to the reductions in fitness by isogenic ΔflaA mutants in most cases when both were tested.
145 Yet, such therapies will even in theory be successful in restoring only a subset of mutants (in these cases, missense and donor splice site, respectively).
Analysis of dEAAT1 transcript levels revealed a reduction in TBPH mutants in all cases examined (n = 7) when compared with w controls (Fig. 7B).
The expression levels were higher with the phenylalanine deletion mutants in 14 cases, slightly lower for three clones and in the same range for the remaining 15 clones.
Several previous studies analyzed differential gene expression of B. anthracis and B. cereus strains and mutants, in some cases exploring the effects of CO2.
Table 3 summarizes the data for all of the mutants analysed, where the CaM-NLS mutants in all cases showed reduced CaM binding, but normal Impβ1 binding, whereas the β-NLS Y127C mutation-carrying SRY fusion protein bound CaM in similar fashion to the WT SRY protein (but showed impaired Impβ1 binding).
