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Furthermore, the enhanced protocol was used to construct mutants in both the gram-negative Escherichia coli BL21 and gram-positive Bacillus subtilis 168 strains.
In this review we describe novel genetic screens for the isolation of fidelity mutants in both Saccharomyces cerevisiae and Escherichia coli RNA polymerases.
Using the classic LacI protein as an example, we describe a genetic selection system using a bidirectional reporter to capture mutants in both allosteric states, allowing the positions most crucial for allostery to be identified.
POR is involved in the production of prolamellar bodies (PLBs), which include carotenoids and Chl precursors in the etioplast (Rosinski and Rosen 1972; Engdahl et al. 2001), as the sizes of PLBs are reduced in the etioplasts of por mutants in both Arabidopsis and rice (Frick et al. 2003; Masuda et al. 2003; Paddock et al. 2012; Sakuraba et al. 2013).
To explore this, we examined phenotypes of single and/or double mutants in both muscle and nervous system development.
To confirm this result we attempted to construct mdh KO mutants in both M. tuberculosis and M. bovis BCG.
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We have used a set of 48 physical, chemical energetic and conformational properties of amino acid residues and computed the difference of amino acid properties for each mutant in both sets of data.
The complemented cheR1 mutants exhibited clearly more reversals as compared with the respective wild-type control and cheR1 mutant in both strain backgrounds (Figure 4E and F).
The transcriptomes of mutants deleted individually in RND-4 and RND-9 (named D4 and D9), and a double-mutant in both efflux pumps (named D4-D9), were compared to that of the wild-type B. cenocepacia using microarray analysis.
The genes up-regulated in 3mlpa mutant in both stages are indicated by asterisk symbol.
We screened for drought and cold response independently and selected the ESKIMO1 mutant in both screens.
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