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The increases in enantioselectivity were higher (up to 5-fold, reaching E = 61) than with mutants identified by random mutagenesis of the entire enzyme.
We have no explanation for the fact that so few mutants identified by us display a defect linked to Sonic hedgehog signaling, as was the case in the screens mentioned above.
(B ) Proportion of REMI mutants identified by iPCR that mapped to exon 1 of gefE locus in each round of selection.
The Con -mutants identified by Skurray [ 14] lacked OmpA, a major outer membrane protein, and resulted in 0.1 to 1% of the parental conjugation frequency.
Thus, it seemed reasonable to expect that many of the mutants identified by our screen would be involved in K+ homeostasis, rather than translation or glycosylation.
Together these data indicate that the two mutants identified by the trichome and seed color phenotypes are two A. alpina Aa ttg1 alleles.
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In this paper, we characterize a SP-A sensitive P. aeruginosa mutant identified by comparative STM, the flagellar mutant flgE.
Overexpression of pax2 has been observed in zebrafish aussicht (aus) mutant, a dominant mutant identified by ENU screening, which exhibit defects in the differentiation of forebrain, midbrain and eyes [29].
Table 2 lists the mutants identified and confirmed by HRM analysis.
In vivo complementation assay of YidC mutants identified as structurally important by MD simulations.
The P. s ojae susceptible (pss) 1 mutant was identified by screening a mutant population created in the Arabidopsis pen1-1 muthat that lacks penetration resistance against the non adapted barley biotrophic fungal pathogen, Blumeria graminis f. sp. hordei.
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