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EPGs recorded from slo-1 null mutants have previously been reported.
Among the 24 Group 1 mutants, only 4 mutants, acrA, acrB, yfgL, and mrcB mutants, have previously been reported to be hypersensitive to beta-lactam antibiotics [28].
S. cerevisiae mre11-H125N mutants have previously been shown to be sensitive to DNA damaging agents including CPT and IR [3], [10], [12], [13]; and a corresponding S. pombe active site mutant (rad32-H134S) is associated with both CPT-sensitivity and IR-sensitivity [8].
The HA-CLR mutants have previously been described (Barwell et al., 2010).
In agreement with this, gas1Δ and ecm33Δ mutants have previously been shown to have a constitutively high level of Slt2 phosphorylation [ 44, 45].
Nevertheless, several clock mutants have previously been shown to alter temperature compensation in Drosophila and in Neurospora (G ardner and F eldman 1981; P rice 1997).
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Isogenic ΔdltA mutants had previously been created in two distinct M1T1 isolates of S. pyogenes.
Both mutants had previously been found to be less likely than wild-type parasites to exit the inoculation site.
As RNAi against ndg-4 caused resistance to replication stress (Fig. S1A), and ndg-4 mutants had previously been shown to be resistant to NDG (Shreffler et al., 1995) and fluoxetine (Choy & Thomas, 1999), we hypothesized that ndg-4 mutants had generally improved stress defense systems.
The sod1Δ mutant has previously been shown to have a prolonged S phase (Carter et al., 2005).
This mutant has previously been shown to bind Ca2+ with kinetic properties similar to those of wild-type CaM, but was unable to activate several CaM-dependent target enzymes in vitro.
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