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Moreover, our data also showed that that both mutants have less capacity of NO binding than wild type Mt-trHbN.
Taken together, the EdU labeling results indicate that the smc-5 (ok2421 ) and smc-6 (ok3294 ) mutants have less nucleotide incorporation, consistent with impaired DNA replication.
As revealed in figure 2, for both collections most mutants belong to the dWD class as 87% (95/109) of the RV mutants and 79% (27/34) of the CE mutants have less than the original 11 WD40 repeats.
The reduced cell elongation in exocyst mutants could conceivably arise because cells in exocyst mutants have less time to elongate in their shorter elongation zones or because they elongate at a slower rate.
Also, this would resolve the paradox that the K161N and R256C mutants have less ubiquitination activity than wild-type when expressed in cells (Chung et al., 2001; Sriram et al., 2005), while possessing normal intrinsic ubiquitination activity in a cell-free system (Hampe et al., 2006).
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The results clearly showed that EBs purified from infected GFP-Rab14wt overexpressing cells were enriched in fluorescent sphingolipids, whereas EBs harvested from infected cells overexpressing both GFP-Rab14 negative mutants had less associated sphingolipids (Figure 8C).
Importantly, the defects in DSB-stimulated translocation in both double mutants had less than a two-fold effect on plating efficiency subsequent to DSB formation, indicating that our determinations of translocation frequency were minimally affected [43].
The genetic background of jasmonate signaling pathway mutants had less effect on WRKY gene expression.
Accordingly, Dyrk1a+/- mutants had less IPs in the SVZ (Fig. 4D).
Cells expressing palmitoylation-impaired mutants had less CD44 in lipid raft fractions and correspondingly more recovered from nonraft domains.
The germ cells in the smc-5 (ok2421 ) and smc-6 (ok3294 ) mutants had less EdU incorporation compared to wild-type, consistent with the requirement for SMC-5 and SMC-6 in promoting efficient DNA replication.
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