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The hair cells from all three mutants have all channels closed at rest and fail to load with FM1-43.
The P. aeruginosa PUPa3 WT strain and all QS genomic null mutants have all been described previously (Steindler et al., 2009).
The yeast Erg25 (sterol 4α-methyloxidase) [44], Erg24 (sterol C-14 reductase) [45], Erg2 (Δ8-Δ7 sterol isomerase) [46] and Erg3 [47] mutants have all been complemented by the corresponding Arabidopsis genes, even though the homology between the yeast and Arabidopsis proteins was fairly low and the natural substrate in Arabidopsis differs somewhat from that of the yeast pathway.
These mutants have all been found to have increased sensitivity to antibiotics and are readily killed by macrophages, leading to attenuated infections in zebrafish models (Cosma et al., 2006; Gao et al., 2006).
As demonstrated in Fig. 4C, the Nde1+/− and Nde1+/−Lis1+/− mutants have all shown clear separation of the MZ and the SP by the migration and invasion of the first cohort of cortical plate neurons by E13.5 (141 41).
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Xylanase activity showed all mutants have an optimum catalytic temperature (Topt) of 55 °C, and with the exception of the S27E mutant, a higher specific activity than the wild-type XynA.
In general, the number of fission yeast mutants produced was ≤ 1% of the total number of barcode clones present in the bacterial sub-libraries used for mutagenesis, so all mutants have a ≥ 95% chance of having unique barcodes.
All seven mutants have a strong defect in the expression of these bradyzoite markers.
In general, all three mutants have a lower degree of lipid-chain unsaturation compared to wild type worms.
Results showed, surprisingly, that all the mutants had an enhancement of biofilm formation with respect to the wild-type.
The docking experiment showed that all mutants had a suitable pattern of interactions, with similar or lower interaction energies compared with the native protein.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com