A broad spectrum of mutants has been obtained including changes in phenotypes such as the flower color, color intensity, flower shape, and petal shape and size.
The intracellular trafficking behavior of these Tf mutants has been assessed by measuring their association with HeLa cells.
The size of the shortened small-t-related polypeptides produced by several of the mutants has been compared with the molecular weight as deduced from the nucleotide sequence.
Sperm whale myoglobin, a protein of broad interest, has been cloned for several years now and a large collection of mutants has been produced.
This usually occurs after several months of therapy, but early detection of lamivudine-resistant mutants has been reported among patients in South Korea.
Based on x-ray crystallography and amino acid sequence data, a panel of putative human ITPase nucleobase specificity mutants has been generated.
The effect of recombination on the emergence of multi-hit mutants has been found to be complex and dependent on the particular scenarios and assumptions considered47,48,49,50.
In the present study, the whole-cell biotransformation using strains expressing CYP BM3 mutants has been evaluated for the stereoselective hydroxylation of the steroid norethisterone (NET), a widely used contraceptive.
To date, investigation of the structural and functional properties of tubulin and tubulin mutants has been limited by the inability to obtain functional protein from overexpression systems, and by the heterogeneous mixture of tubulin isotypes typically isolated from higher eukaryotes.
The generation of conditional mutants has been an effective approach to studying bacteria and validating drug targets, and mutants of Mycobacteria are no exception.
Further, expression of these genes in Fe uptake-defective yeast mutants has been demonstrated to reverse growth defect associated with Fe uptake inability (Li et al. 2013; Pedas et al. 2008).
