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FOR about twenty years it has been known that the yield of induced mutants has a different type composition in differing mutagenic conditions1.
Second, by comparing results from the linker constructs, the general trend is that C-terminal truncation of the donor (the CFPΔ/GFPΔ mutants) has a beneficial effect on FRET efficiency, particularly when introduced in constructs exhibiting moderate basal E values.
Interestingly, each of these mutants has a normal carotenoid content, and energy transfer to B850 pigments is unaffected [43].
CFU counting of THP1 cell lysates indicated that none of the early granuloma mutants has a severe replication defect in host cells (Fig. 3A).
To the extent that longevity extension in dwarf mutants has a common basis with longevity extension by CR, these three genes are the most well supported longevity-associated genes identified by this analysis.
In fact, the monocotyledonous phenotype of rpk1 mutants has a maximum penetrance of ca. 10%% [ 21, 22], which could be elevated by adding mutations in the related TOAD2/RPK2.
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In the second group, mutants have a typical defense defect in which bacterial growth is increased and survival is decreased.
Swine receiving the rΔ727 813 mutants had a significant decrease in lymph node enlargement compared to rVR-2332.
This is only likely to occur in small populations where deleterious mutants have a chance to reach fixation.
After 48 h in synthetic wounds, (Figure 5), both mutants had a relative fitness of 1 regardless of culture condition.
These well-defined mutants, having a selective disadvantage for growth, would not have been readily obtained by conventional methods used to screen for viral mutants.
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