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que mutants harbor abnormal amino acid levels, similar to MSUD patients and consistent with an error in branched-chain amino acid metabolism.
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Liu et al. reported that the cancer cells usually harbor abnormal metabolic status [ 48].
These mutants harbor a gain of the function mutation, T403A, in the Hr gene.
Consequently, B9 inhibits the proliferation of tumor cells harboring abnormal activation of STAT3, such as, MDA-MB-468, MDA-MB-231 and DU145.
Mutants resistant to nitrofurantoin and trimethoprim displayed lower fitness effects, and several resistant mutants harbored apparent cost-free resistance mutations (Supplementary Fig. 4).
However, the mutants harboring these mutations showed increased MICs (minimum inhibitory concentration) as compared to wild type strains.
Grey bars Sup mutants, white bars Sup mutants harboring pRRN5.
Grey bars ∆fob1Sup mutants, white bars ∆fob1Sup mutants harboring pRRN5.
b Intracellular total RNA content of nine Sup mutants harboring pRRN5 plasmid.
Figure 3 Southern blot analyses of the argB -auxotroph mutants harboring different lengths of direct repeats for marker recycling.
In the next step, we measured the copy number of rDNA repeats in Sup mutants harboring pRRN5 plasmid.
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