Results showed that all deletion mutants grew less robustly than full-length recombinant virus, yet all but the large deletion virus (rΔ324 726) recovered to parental viral RNA levels by study end.
However, we found that cdc13-1 pif1Δ dna2Δ mutants grew less well than cdc13-1 pif1Δ mutantSupplementaryary Figure S2A).
However, it was clear that cdc13Δ exo1Δ pif1Δ mutants grew less well than CDC13+ exo1Δ pif1Δ mutants on the tetrad dissection plates.
cdc13-1 expressingins expressing Exo1-Tap or mutants grew less well than cdc13-1 exo1Δ carrying the empty vector pRS413 at 28°C (data not shown).
This model predicts that singly mutant cells should grow less well on lactose than those carrying two mutations.
Peroxisomal mutants that inefficiently perform β-oxidation fail to germinate or grow less vigorously [ 2, 3].
Arabidopsis pex mutants β-oxidize fatty acids inefficiently and therefore fail to germinate or grow less vigorously.
Both of these strains grow less well than wild-type, suggesting that nutrient sensing and/or utilization abilities are compromised in the mutants.
"May your shadow never grow less".
And it's going to grow less.
