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GI confers a weak late flowering in field conditions of 7 10 days during summer while vin3-1 mutants generate a longer delay of 25 50 days (Sung and Amasino, 2004; Wilczek and et al., 2009).
The cell adhesion molecule N-Cadherin may play a key role in interdigitation and intercalation as N-Cadherin mutants generate a complex neuroepithelial organization that time-lapse analysis suggests could result from defective interdigitation of superficial and deep layers [ 10].
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Interestingly, the vac17Δ tor1Δ mutants generated a new vacuole in the daughter cells.
The Skm1 mutants generated a significant linkage (LOD score = 3.612) at chromosome 15 at marker rs13482455 (16.73 Mb).
Although most of these properties were not significantly different between wild-type and mutant larvae (Table 1), que mutants generated a significantly greater number of bouts following touch stimulus applied to the head than did wild-type larvae [11.6±6.2 bouts and 1.9±0.9 bouts, respectively (t=3.5, P<0.01, n=5)].
When expressed in HEK293 cells, this Ano6 mutant generated a small but significant whole-cell Cl− current (ΔI=0.82±0.13 nA; n=5) upon activation by ionomycin (1 μM).
In the binding area formed by helix1/helix4+loop we constructed a F18A mutant, generated a combined D19N-L20A-P21A mutant and a combined P22A-A23S mutand, and R70Q, W74A and Q78A single mutant molecules.
These large MVP rolls suggest that the 6-His-MVP mutant generates a structure that represents a vault assembly intermediate, rather than just a chaotically misassembled swirl of protein.
Thus, A236G EIZS generates a mixture of 70% epi-isozizaene (18), 2% zizaene (17), and 28% of a mixture of four unidentified sesquiterpenes with a near-normal catalytic rate, whereas the corresponding A236F EIZS mutant generates a mixture of 77% epi-isozizaene (18), 15% zizaene (17), and 8% β-cedrene (12) with a nearly 2000-fold reductinn in kcat.
The vte1 npq1 mutant was crossed with the pdx1 single mutant to generate a triple mutant (vte1 npq1 pdeficientcinnt in vitamins E and B6 and in zeaxanthin.
Helix-forming propensities were averaged across all the fifty mutants to generate a single value representing the mutated mRNA helix-forming propensity.
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