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The result showed that the truncated and Q to L mutants gave higher and considerably lower number of the cells than unmodified S-Mpl, respectively.
For CHO-K1, U2OS and 17.1 cells, all the three mutants gave higher transduction efficiencies than the WT (wild-type) virus.
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The normally spliced mutant TPO transcripts gave higher level of expression than the alternatively spliced TPO transcript in the ratio of 10.6 : 1 in normal area of thyroid tissue and 12.5 : 1 in lesionic area of thyroid tissue.
In contrast to the truncation mutants, two TLS mutants expressed CAT activities comparable to those of the wild-type background (TNP), and three TLS mutants gave significantly higher activities than strain TNP.
The normally spliced mutant TPO mRNA transcript gave higher level of expression than the alternatively spliced TPO transcript in the ratio of 9.9 : 1 in normal area and 9.5 : 1 in lesionic area.
In agreement with the microarray data, alpha crystallin, EspB, and Hsp65 gave higher expression in the wild type whereas ICL gave higher expression in the phoP mutant (Figure 2 and Table S2).
Progressing sequentially through increasingly higher doses of antibiotic, low-resistance mutants gave rise to moderately resistant mutants, eventually spawning highly resistant strains able to fend off the highest doses of antibiotic.
Furthermore, the Δ399-Δ406 double mutant gave the highest receptor-Kras BRET signal of the Venus-tagged double mutants, which may account for the receptor-arrestin BRET signals for this mutant being higher than observed for wild type OX2 receptor.
Interestingly, the affinity of wtCXCL8 for HS was 5-fold higher compared with the protein's affinity for heparin, and again all 17/21 mutants gave slightly lower affinities (compared with WT) with the exception of the F21R mutant (Table 2).
Together with the high density of myoblasts observed in the tw mutant (Fig. 7F), these results suggest that both epidermal cells and muscle progenitor cells of Drosophila POMT mutants give rise to cell adhesion derangement.
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