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Passaged MAMM mutants from two independent trials were plaque purified (Fig. 6B, N = 9).
Next, we collected 163 MS mutants from two rice databases: Oryzabase and China Rice Data Center [ 15, 16].
Several prp3 vti13 double mutants from two independent crosses were characterized for their patterns of root hair growth in these studies.
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We evaluate the stability changes upon mutations for 595 mutants from five structurally unrelated proteins, and find significant correlations between the predicted and experimental results.
In this study, we characterized a panel of more than 20 cytosolic thermosensitive mutants from six essential genes in Saccharomyces cerevisiae.
Additionally we performed a flg22 time course on mutants from three major hormonal pathways (npr1-1 – sacidylic acid [ 69], jar1-1 – jacidnic acid [ 70] & ein2-5 – ethylene [ 71]).
To evaluate the diversity of mutations in the insertion mutant library, four genetic screens were performed on 3600 mutants from ten 384-well plates for phenotypes that could be easily scored.
The growth kinetics (in liquid medium) of wild-type and calcineurin mutants from three C. gattii molecular type backgrounds were similar at 24°, whereas C. neoformans calcineurin mutants exhibited a slightly slower growth rate compared with the wild-type or complemented strains.
Although swim bladder inflation was delayed at 5 days post-fertilization (dpf), no overt evidence of edema or compromised circulation was detected morphologically at 3 5 dpf (stages when OPC defects were apparent among progeny) in notch3 st51/st51 or notch3 zm/zm mutants from five independent heterozygous incrosses.
As shown in Fig. 1D (Table S1), the patterns of gene expression one day post infection with either wild type PA14 or the gacA mutant from two independently conducted experiments were essentially identical.
We also simulated PAC detection of recurrent mutations with two breast cancer cell lines, of which HCC1937 had skipped RB1 exon 22, and we were already able to identify the mutant from among two samples up to even five mutants from among six samples (Fig. 4B).
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