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The lowest proportion of mutants from the Ottenby data set was 4.35% for the N3 subtype, which was still higher than the proportions of mutants from all other avian subtypes from the NCBI data set, except for N5 (Table 2).
The data indicated that Emm-negative mutants from all of the pattern A serotypes (i.e. M1, M5, M6, and M24) were deficient in their ability to form biofilms (Fig. 4).
The 2,3-BD production increased significantly in the pta and adhE mutants from all carbon sources, except lactose.
We found that C. gattii calcineurin mutants from all three molecular genotype isolates were avirulent or strongly attenuated for virulence (P < 0.0001 as compared to each wild type; Figure 4A).
Specifically, we examined the membrane traffic mutants from all three classes identified in the original screen by asking whether growth on medium containing increased KCl suppressed the carboxypeptidase Y (CPY) secretion phenotype exhibited by these strains.
We are now generating mutants from all the sRNAs for functional analysis (to be published separately).
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To provide a more intuitionistic view of how these mutations may affect the structure of MxA, we performed modeling of several mutants selected from all four groups, and compared the output models to the original crystal structures.
To examine whether this could account for the striking differences in the tumor growth phenotype (Fig. 1), we normalized the tumor volumes to relative expression levels of wild type or mutant protein from all available experimental data.
In stark contrast, mutant lines from all three geographic populations showed a significant decline in fecundity of up to 47 percent after between two and four generations.
As shown in Figure 2A, considerable amounts of Swi3 mutant proteins were recovered from all mutants except for swi3-E10.
Consistent with the IF analysis, protein levels were reduced in the heterozygous mutant and absent from all independent homozygous mutant clones.
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