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Combining the proline mutations with rationally designed mutants from a previous study led to 2400-fold increase in the affinity of the A6 T-cell receptor for Tax-HLAA2.
The results obtained showed that the adeA gene served as an efficient selection marker in developing a novel host-vector system with quadruple auxotrophy in A. oryzae, thus providing a powerful tool to breed multiple auxotrophic mutants from a deuteromycete wherein sexual crossing is impossible.
The robot then plans and executes experiments to test its hypotheses--selecting yeast mutants from a collection, incubating cells, and measuring their growth rates.
In this study, we report the first application of Agrobacterium tumefaciens-mediated transformation (ATMT) for construction of insertional mutants from a virulent defoliating isolate of V. dahliae (V592).
asc1Δ and its parental strain were transformed with plasmids that promote the expression of an inactive (Pkc1K853R) or a constitutive-active (Pkc1R398A) kinase mutants from a galactose-inducible promoter [32].
All of the mutants from a single animal were pooled together in a single microtube.
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Isolation of rec- mutants from an F-prime merodiploid strain of Escherichia coli K-12.
The HLA-A2 antigen-specific monoclonal antibody BB7.2 and complement were used to immunoselect mutants from an ethyl methanesulfonate-mutagenized human B lymphoid cell line, T5-1.
All seven HIV-1 RT mutants from an AZT-resistance pathway (Figure 1) were generated by shuffling PCR.
We recovered double-homozygous mutants from an rst+/− ;eis+/− incross at the expected 1 16 ratio for two independently segregating Mendelian mutations (n=8/130).
I am not some misunderstood mutant from a comic book or a horror movie, although some have treated me as such.
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