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The L19F and R164Q mutants formed a subcluster within cluster 2 suggesting, consistent with our foci formation data, that these mutants are phenotypically distinct from the G13D, A146T and K117N mutants.

All mutants formed colonies in the presence of cyclohexane, although the colony-forming efficiencies differed among the mutants.

As for the Walker B mutants, both 1E/ Qand 2E/Q mutants formed closed rings at ~70% (Fig. 3a, b, Supplementary Figure 14c).

Wild-type tail fragments formed ordered paracrystal arrays, whereas mutants formed aberrant aggregates.

The Ia intermediates of the W7F, W7F/M131C, and W7F/L135C mutants, which are formed within the burst phase, all have high fluorescence intensity.

As expected, both mutants form a stable complex with YfiR.

The acga- mutant formed normal aggregates and fruiting bodies (Figure 3A).

The ap1 cal double mutant formed many floral primordia at the first and second developmental stages.

Amyloid formed within a restricted pH range.

We have shown that IDH1 WT WT, WT:mutant, and mutant:mutant dimers form in cells that express both mutant and WT forms of IDH1 (Fig. 1b).

We showed that parasites expressing a mutant form of AMA1 within the cytosolic tail were fully competent to secrete the content of the rhoptries and to form a MJ, and as such are intimately attached to the host cell.