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On RDC medium, ΔMovam7 mutants formed a thin white colony that gradually collapsed into a film-like material (Figure 3A).
Importantly, all three single mutants formed a forebrain that was near normal in size, although its patterning was affected (Fig. 7E H and data not shown).
As can be seen in these AFM images, the W10I and Y131I mutants formed a lattice.
The S32A and S32D mutants formed a complex with glucokinase to the same extent as wild-type (Fig. 6C).
Although a small percentage of the double mutants formed a few sperm, they did not produce any oocytes.
As predicted by our model, early-stage mec-8; pha-1 e2123); pha-1 e2123e mutants formed a stereotriplel keyhole, consistent with the presence of a pharyngeal-epidermutantsnection.
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As expected, both mutants form a stable complex with YfiR.
Interestingly, yopK mutants form a larger pore and in line with this notion, yopK mutants overtranslocate Yop effectors [24].
GFP-Slimb-Δ-F-Box reproduces Osk localization in slimb mutants, forming a broad posterior crescent.
It is possible that the N-terminal deletion mutants form a dimer interface different from that of wild-type SecA.
By 26 hpf, eis mutants form a single large otolith that is initially untethered and misshapen but biomineralised.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com