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Using published guard cell-specific transcriptome data, we identified a panel of pectin-modifying genes that are up- or down-regulated in Arabidopsis thaliana guard cells, and examined stomatal development and function in homozygous T-DNA knockout mutants for these genes.
It was necessary to use a reduction strategy to the generation of mutants for these models.
The OsLOL2, OsLSD1, OsSpl11, and OsSpl7genes are negative regulators of cell death, and mutants for these genes display spontaneous lesions resembling disease.
Inhibition of IGS transcription by DDM1 and MET1 would explain why IGS siRNAs overaccumulate in mutants for these proteins.
Therefore, we next created mutants for these ETS1 sites and compared the luciferase activity of the −108CIP2ALuc constructs harboring the mutations to that of the wild type.
Bimodality is apparent without added glucose in all loss-of-function mutants for these G protein components, suggesting that the heterotrimeric G protein complex attenuates the bimodality and that glucose inhibits this attenuation through the complex.
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As the [−2A]N-TIMP-3 mutant was still active against the ADAMTSs, the Ki (app) values of these mutants for the various metalloproteinases were determined.
Q169A was chosen as a representative mutant for these experiments since this substitution was the least detrimental to telomerase activity in vitro.
We find that, although single mutants for Sans or crinkled are adult viable [29] (this study), animals double mutant for these genes do not develop to adulthood (data not shown), underscoring the developmental role of Usher syndrome orthologs in Drosophila.
The overall morphology of embryos either double or single mutant for these genes was identical and the distributions of FasIII and Dlg were also indistinguishable in the three mutant backgrounds (Fig. 4G–I').
If cdc-42 is a direct target of mir-34 and mir-83, we would expect its expression to be elevated in animals mutant for these two miRNAs.
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