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If this were the case, then although removing either PC is insufficient to induce Daf-c on their own, they should modify Daf-c penetrance in a sensitized background, such as lf mutants for functionally redundant ILPs.
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First, mutants deleted for functionally related genes did not always exhibit the same phenotypes; for example, of the two endosome genes, the atg20Δ mutant was sensitive to H2O2, while the shd1Δ mutant was resistant.
MapMan is a useful tool to provide global views of diverse aspects of high-throughput data and can be used to functionally classify rice genes with flanking sequence tag (FST) mutants for systematic functional studies (Thimm et al. 2004; Usadel et al. 2009; Jung and An 2012).
ADC1 and ADC2 are functionally redundant as mutants for either gene are viable but double mutants are not [ 38].
He etablished new protocols for functionally connecting the two systems.
The mutants were functionally evaluated using the plate based dual complementation-cum-toxicity assay.
This ΔhtsAΔisdE mutant is functionally inactivated for both Isd and Hts-mediated heme transport.
To functionally characterize Hrk1, we constructed targeted deletion mutants for HRK1 in both wild-type and hog1Δ mutant strain backgrounds and performed an array of phenotypic analyses in comparison with the hog1Δ mutant.
In contrast, all four pUL31 ZNF mutants could not functionally substitute for WT pUL31.
To functionally characterize the multiple interfaces on CRYs mapped by the crystal structures, we systematically assessed several representative CRY mutants for their abilities to rescue rhythmicity in Cry-deficient MEF cells.
Here we show that the HSF1 S303A mutant functionally complements for the lost of yeast HSF (Figure 1A, B).
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