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We performed genotyping to confirm the T-DNA insertion in the Z3 gene, and obtained homozygous mutants for each allele (Additional file 5: figure S5f).
To elucidate to what extent each enzyme encoded by the SSGs, including SSIIa, is rate-limiting for starch synthesis in the endosperm, we need to develop and analyze a series of IR36 mutants for each SSG.
Regarding the numbers of mutants for each group of operators (columns four to six in the table), we can observe that changes applied to pointcuts (i.e. operators from G1 group) yield the largest number of mutants for all systems except for FactorialOptimiser.
A scatterplot of fold-change in dcl1-7 and hen1-1 mutants for each target was generated.
We therefore proceeded to evaluate the essentiality of each RNR class during the infectious process, first constructing nrd mutants for each RNR class and then combining them to obtain double mutants (Materials and Methods).
Basal expression levels of Diptericin, Drosomycin, Defensin (Figure 4C, D) in whole animals and of Diptericin, Drosomycin3 and Defensin (Figure 4E, F) in guts were not significantly different in controls and Toll-9−/− mutants (for each experiment, P≥0.06).
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Moreover, the TGase production of the best mutant for each round presented a fluctuant improving trend.
To determine if smf-1, smf-2 and smf-3 are involved in Mn uptake and toxicity in the worm, we made use of available deletion-mutants for each of the three genes.
A panel of homozygous Arabidopsis plants mutant for each AGO protein was screened for hyper-susceptibility to TCV, a positive strand RNA virus in the genus Carmovirus.
To uncover the roles of specific AGOs in limiting virus accumulation we inoculated turnip crinkle virus (TCV) to Arabidopsis plants that were mutant for each of the ten AGO genes.
One representative mutant for each identified crtB insertion site is shown.
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