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We assessed slc mutants for additional phenotypes.
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Using this method we have subsequently identified multiple deletion mutants for three additional wheat genes putatively involved in plant defence.
To further examine the hINSC96Y-dependent interaction of sfl, we examined RNAi knockdowns and mutants for two additional genes in the HS biosynthetic pathway, ttv and botv, producing the glycosaminoglycan polymer that is modified by sfl (Lin 2004).
We also included the deletion mutants for an additional five yeast genes (HRK1, TPO3, MET7, PNP1 and GPD1), which are HP and whose products are orthologous to specific cancer-drug targets.
We are also continuing to screen the heterozygous mutant zebrafish for additional cancer-prone lines and have established a second screen to identify genes that are essential for cell cycle regulation.
These mutants were chosen for additional analysis because they were found in more than one independently-isolated Env DNA fragment and thus they could not have resulted from an error during PCR amplification.
Recently, various studies have examined multiple Bbs mutants in parallel for additional phenotypes.
To minimize the sequencing cost and maximize the putative applied outcome, the eight control individuals were chosen as being homozygous mutant for two additional distinct recessive diseases (four mutants each) for which the chromosomal location was known but the causative genes and mutations were still to be found.
Mutant CFPs selected for additional characterization were transformed into M15 pRep4) bacteria for protein production.
We performed genotyping to confirm the T-DNA insertion in the Z3 gene, and obtained homozygous mutants for each allele (Additional file 5: figure S5f).
A screen for additional mutants of SH1000 that were defective for survival in the presence of linoleic acid identified several Tn917 transposants from a 5,000 clone library of mutants.
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