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Although FLC was so highly expressed in clf;fca, the double mutants flowered much earlier than fca (Figure 3B).
Although the cfm4 mutants flowered much later than the wild-type plants, the size and number of leaves at the time of bolting were not different between the wild-type and mutant plants, suggesting that CFM4 does not affect control of flowering time.
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For example, Arabidopsis FT has the closely-related paralogue TSF and double ft tsf mutants flower much later than the single mutants [ 1, 11].
Under non-inductive short-day (SD) conditions, all transgenic plants flowered much earlier than WT and the ft-10 mutant, both of which did not flower until 60 days after sowing in soil.
Both NILs flowered much earlier than NY7.
The T3 lines flowered much more rapidly than the control amiR-FT plants in both conditions.
Following DEX application, all the TG plants flowered much earlier than non-treated plants.
Vernalized atvoz1-1 atvoz2-1 plants flowered much earlier than their non-vernalized counterparts.
The z3 mutant exhibits a pleiotropic phenotype in addition to leaf variegation; the z3 mutants flowered late compared with the wild type (Additional file 1: figure S1a-b).
The brm mutants flowered with less leaves than WT plants, but a percentage of the mutant plants never flowered under SD [8].
Furthermore, when we compared the flowering times between atjmj4-1 single and atjmj4-1 flc-3 double mutants both in LD and SD, the double mutants flowered earlier than the single mutants in both photoperiodic conditions (Figures 3A and 3B).
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