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Creation of attenuated mutants, expression of potential antigens in live vectors, and purification and direct synthesis of antigens in new systems have immensely improved vaccine technology.
In contrast, in hypomorphic CSN5 mutants, expression of the CSN5 protein would often allow to complete the Cullin3 phase, leading to an "intermediate" branching phenotype.
In both the whirler and shaker2 mutants, expression of gelsolin is considerably reduced at P2 and is ablated from P5, similar to that seen for p55 [11] (Fig. 3).
In the absence of TCF (pan mutants), expression of Wg along the ventral midline had no visible effect on the polarity of the denticles (results summarized in Table 2).
The cells were co-transfected with reporter plasmids containing binding sites for TCF [29] (see Materials and Methods), along with wild type and enzymatically inactive UCH L1 mutants (expression of UCH L1 proteins was confirmed by western blot with HA antibody, Fig. 2D, top panel).
In other mutants, expression of axial tissue and dorsal foregut endoderm markers was maintained despite loss of Foxa2 from ventral foregut endoderm (Fig. 6I J).
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In sfr6 mutants, expressions of KIN1 and COR15A, which contain the CRT/DRE motif in their promoters, were upregulated, as also seen in 35S::LOV1 plants.
In the degS S76D mutant, expression of comK was 5-fold lower, and not induced at all in the transition phase (figure 4C).
In the background of a levQ (sp) deletion mutant, expression of the PfruAΔcre-lacZ promoter fusion (BSCZ) [9], which requires LevR for activation but lacks the CcpA binding site (CRE), was reduced to background levels.
Moreover, in the prg-1 mutant, expression of ~87% of the miRNAs did not differ significantly during development.
However, while Dd-dmtA expression restored the DIF-less phenotypes in the dmtA mutant, expression of As-dmtA did not, (Fig. 4A,B; supplementary material Fig. S3A).
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