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Mutants expressing a deletion of the proline-rich domain alone and in combination with the SH3 domain were also capable of transforming hematopoietic cells to factor independent growth.
We demonstrate that mutants expressing a truncated P110 lacking the last thirty amino acids and obtained by homologous recombination show levels of P110 and P140 closer to those exhibited by the WT strain.
To determine whether mutants expressing a single VPg could also manifest a defect in cell entry, the capacity of mutants V19-4 and V3 expressing either wild type 2C or 2C with R55W to enter cells was determined.
To compare intracellular protein synthesis directed by the mutant FMDVs, proteins were metabolically labeled between 3 and 4 hours post electroporation. BHK 21 cells infected with mutants V3, V19 4 and V15 9 encoding R55W in 2C presented modestly higher levels of viral proteins than the same mutants expressing a wild type 2C (Figure 7).
Drosophila mutants expressing a Ph protein that lacks the O-GlcNAcylated S/T stretch precisely reproduce the phenotype of Ogt mutants (Gambetta and Müller 2014).
Left: sleep profiles, with standard error, of rye mutants and mutants expressing a UAS construct of the putative rye cDNA under control of its own promoter (ryeP-Gal4) in a 12 12 LD cycle.
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A VACV mutant expressing a variant of VCP lacking cell surface localisation resulted in an attenuated phenotype similar to that of the VCP deletion mutant after i.n.n
A mutant expressing a truncated SsrA tag was viable in H. pylori, but affected in competence and tolerance to both oxidative and antibiotic stresses.
Furthermore, citrus canker symptoms could be observed in infections of the ΔhrcU mutant expressing a HrcUXAC variant in which the NPTH site has was abolished.
To further confirm that the expression of a truncated P110 does not lead to a MTO phenotype, a M. genitalium mutant expressing a P110 derivative lacking the last 30 amino acids was obtained by homologous recombination (Fig. 8A).
To corroborate this conclusion an H. pylori mutant expressing a derivative of ArsS with a substitution of H94 to alanine was constructed which also exhibited reduced pH-responsive transcription of hp0119 and hp1432 (Fig. 2 and data not shown).
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