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All mutants, except that encoding only VPg1, were replication-competent.
Inverse KSIE kcat/ Km) values were observed with varying magnitudes for all hydroxylase mutants except that of Q228A.
We also found that all mutants (except that M42C is slightly off) give Ef,o measurements compatible (within error) with the final model.
Within differentiation defective tissue, the xylem cells were generally similar to those of bp single mutants except that the relatively few cells that developed secondary walls were extremely small compared with those of bp.
The chain-length profile of ss1 L /be2b was almost identical to that calculated by adding the profiles of the ss1 L and be2b single mutants, except that the molar of very short chains with DP 6 7 did not increase in the pattern generated from ss1 L /be2b-WT profile.
Fis mutants were evaluated for activation of Hin-catalyzed DNA inversion in vivo using the same lacZ reporter system as utilized for the Hin mutants, except that the Fis mutant gene on a plasmid was introduced into the cell containing Hin-wt (see Safo et al., 1997 for experimental details).
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Spectral changes upon substrates addition are similar to the ones observed with the T254A mutant, except that a residual absorbance is shown in the 420 430 nm region.
The binding mode of the Mg2+3 PPi cluster is generally unchanged in each mutant, except that the PPi anion rotates slightly in F95M EIZS such that the bridging oxygen atom moves ∼0.6 Å, and the Mg2+C ion moves ∼1.0 1.5 Å in the F95M and W325F mutants.
A similar strategy was utilized for crossing the eat-2 ad465 eat-2 ad465nto the daf-2 mutationackground, except that recovered F2 dauers were screened for slow pump rates (<60 ppm intoathe(ad465) vs >200 ppm in the daf-2 mutants at 15°C) to identify animals homozygous for the eat-2(ad465) mutation.
All these mutant residues, except that at NA-91, are located in the viral functional domains, suggesting that they may play roles in the human adaption and virulence of 2009 H1N1pdm.
In fact, circumstantial evidence supports the possibility that these mutants may indeed be in a biologically relevant conformation given that all the mutants (except for G336V that did not overexpress) could be purified using the very mild detergent LMNG.
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